Journal: Cell Transplantation

Article Title: Impact of One Versus Two Consecutive Doses of Endothelial Cells (EPCs) and EPCs-Derived Condition Medium on Protecting Myocardium from Acute Ischemia-Reperfusion Injury in Rat

doi: 10.1177/09636897211007049

Figure Lengend Snippet: Protein expressions of apoptotic, fibrotic, mitochondrial-damaged, and pressure overload/heart failed biomarkers in LV myocardium by day 3 after IR induction. (A) Protein expressions of cleaved caspase 3 (c-Csp3), * vs. other groups with different symbols (†, ‡, §, ¶), P < .0001. (B) Protein expression of cleaved poly (ADP-ribose) polymerase (c-PARP), * vs. other groups with different symbols (†, ‡, §, ¶), P < .0001. (C) Protein expression of Smad3, * vs. other groups with different symbols (†, ‡, §, ¶, λ), P < .0001. (D) Protein expression of transforming growth factor (TGF)-ß, * vs. other groups with different symbols (†, ‡, §, ¶), P < .0001. (E) Protein expression of cytosolic cytochrome C (cyto-CytC), * vs. other groups with different symbols (†, ‡, §, ¶), P < .0001. (F) Protein expression of brain natriuretic peptide (BNP) * vs. other groups with different symbols (†, ‡, §, ¶), P < .0001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test ( n = 6 for each group). Symbols (*, †, ‡, §, ¶) indicate significance (at .05 level). Group 1 = sham-operated control; group 2 = ischemia-reperfusion (IR) + culture medium; group 3 = IR + endothelial progenitor cells (EPCs) administered at 3 h after IR induction; group 4 = IR + EPCs administered at 3 h and 24 h after; group 5 = IR + condition medium (CM) administered at 3 h after IR procedure; group 6 = IR + CM administered 3 h and 24 h after IR procedure.

Article Snippet: The membranes were incubated with the indicated primary antibodies [CD31 (1:1000, Abcam, Cambridge, UK), von Willebrand factor (vWF) (1:1000, Abcam, Cambridge, UK), endothelial nitric oxide synthase (eNOS) (1:1000, Abcam, Cambridge, UK), cleaved poly (ADP-ribose) polymerase (PARP) (1:1000, Cell Signaling, Danvers, Ma, USA), cleaved caspase 3 (c-Csp3) (1: 1000, Cell Signaling, Danvers, Ma, USA), Smad3 (1:1000, Cell Signaling, Danvers, Ma, USA), brain natriuretic peptide (BNP) (1:500, Abcam, Cambridge, UK), NADPH oxidase (NOX)-1 (1:1500, Sigma, St. Louis, MO, USA), NOX-2 (1:500, Sigma, St. Louis, MO, USA), cytosolic cytochrome C (cyto-CytC) (1:2000, BD, Franklin Lakes, NJ, USA), mitochondrial cytochrome C (Mito CytC) (1:2000, BD, Franklin Lakes, NJ, USA), p47phox (1:1000, Merck Millipore, Burlington, Ma, USA), transforming growth factor (TGF)-ß (1:5000, Abcam), vascular endothelial growth factor (VEGF) (1:1000, Abcam, Cambridge, UK), CXCR4 (1:1000, Abcam, Cambridge, UK), stromal cell-derived factor (SDF-1α) (1:1000, Cell Signaling, Danvers, Ma, USA) and hypoxia-inducible factor (HIF)-1α (1:1000, Abcam, Cambridge, UK) for 1 hour at room temperature.

Techniques: Expressing